Larva a small to medium plumosus-type. Gula and frontoclypeus darkened, although variable and frontoclypeus may be pale (Spies et al. 2002). Ventral tubules about length of segment VIII, posterior pair coiled, shorter than described by Fittkau for South American specimens. Those of Kansas specimen are also longer. Mentum (Fig. c) with somewhat rounded teeth; centre tooth relatively narrow and rounded, c2 teeth moderately well seperated, with reduced 4th lateral. Pecten epipharyngis (Fig. a) with about 12 somewhat irregular teeth. Mandible (Fig. e) with 3rd inner tooth relatively well developed (type II). Antenna (Fig. b) with relatively narrow basal segment, about 3.3 times as long as wide; AR about 1.6 - 2.05; A2/A1 about 0.22 - 0.28; A4/A3 about 1.23-1.82.
Cytology
: 4 pairs of chromosomes with the pseudothummi arm combination AE, BF, CD, G.
Arm G relatively long, closely paired with a nucleolus about 1/4 from one end and a Balbiani ring near the other end. No nucleoli in other chromosomes. Moderate amount of heterochromatin at the centromeres. Only a small inversion in arm A of the Kansas specimen has been observed in North American samples, but polymorphism for arms A, B, and C is present in Central or South American samples.
cag A1: 1a-e, 9a-e, 2d-3b, 8g-d, 1k-f, 3c-i, 13-15, 4-8c, 2a-c, 10-12, 16-19
cag A2: 1a-e, 9a-e, 2d-3b, 8g-d, 1k-f, 3c-i, 13-14, 8c-4, 15e-a, 2a-c, 10-12, 16-19 ie. as sp.Villa Paz
cag A3: approx 1a-e, 9a-e, 2d-3b, 8g-d, 1k-f, 3c-i, 13a-15e, 4a, 6b-4b, 6c-8c, 2a-c, 10-12, 16-19
cag B1: Bulb and distal dark bands about 1/3 from end of arm.
cag B2: Inversion of about half of arm near distal end.
cag C1: Typical band group 3-4 about 1/3 from centromere.
cag C2: Inversion of about central half of arm, moving group 3-4 to about middle of arm.
cag E1: 1-3e, 10b-9, 3f-8, 12b-10c, 12c-13
cag F1: 1-6b, 19-18, 11f-14, 17-15, 11e-6c, 20-23 i.e. as in sp. WOC.
Click here for the polytene chromosomes
Found: California - Whittier and Coyote Creek, Los Angeles Co.; Huntington Beach,
Orange Co.;
Good Samaritan retirement home, Corona, and Valley Seminary District, Riverside Co.
Florida - Winter Haven, Polk Co.
Kansas -Nill Creek, nr Craig, Johnson Co.
Also Panama, Colombia, Peru
C. calligraphus in North America appears to be a complex of at least two species. Both are present at the type locality at Belem, Brazil, but the form with the described chromosomes appears to be less frequent. Referred to in some published work as 'Sp. 52' or 'Coyote Creek species'. Wülker & Morath (1989) give the sequence of arms A, E and F; and larva and cytology are described by Spies et al. (2002). A form with a different karyotype has been found in Argentina, but this form has not been found in North America.